We thank Dr. Basler lab, Dr. Bellen lab, Dr. Calos lab and Dr. Perkins lab for allowing our services to use the attP lines generated in their labs. We also thanks Bloomington Stock Center for providing all these lines.

Reference: 
P{CaryP} insertions: Groth et al. 2004
PBac{y[+]-attP} insertions: Venken et al. 2006
M{3xP3-RFP.attP}, M{vas-int.B} and P{nos-phiC31\int.NLS} insertions: Bischof et al. 2007 (see FlyC31 for additional information the phiC31 integrase system)
M{vas-int.Dm} insertions: K. Basler and F. Karch, FlyC31
P{attP.w[+].attP} insertions:  Bateman et al., 2006

We provide micro-injection service for almost all the phiC31 lines listed in Bloomington Stock Center (table below). The service includes injection only service (service A) and extended service for screening the transformants (service B).

Service A: injection of 150 to 200 embryos of selected line, sending back 50 to 100 survival larvae with service report.

Service B: including service A, plus setting up 30 to 50 crosses, sending back transformants with service report.

The price for service A: $180 plus $25 handling charge per order. If you need inject into the lines other than our collection, please provide two bottles of flies. You will be charged for extra $25 fly handling fee per order.

The price for service B: $280 plus $25 handling charge per order.

The charge for all the service is non-refundable. However, if there is no transformants, we provide one free re-injection.

The quality of DNA is critical for successful transformation. We recommend using Invitrogen Purelink Hipure Plasmid Filter Maxiprep kit for large DNA (size over 20kb) preparation, and Qiagen Midi or Maxi kit for size less than 15kb DNA preparation. Dissolve DNA in DEPC treated water with concentration over 1.0ug/ul.

RTF standard attP lines collection 

Bloomington Stock #	Symbol	Cytology	RTF order number	Survival rate	Transformants
24480	M{vas-int.Dm}ZH-102D, M{3xP3-RFP.attP}ZH-2A	102D, 2A3	24480 or 2A	30%-50%	+++
24481	M{vas-int.Dm}ZH-2A, M{3xP3-RFP.attP'}ZH-22A	2A3, 22A2	24481 or 22A	30%-50%	+++
24482	M{vas-int.Dm}ZH-2A, M{3xP3-RFP.attP'}ZH-51C	2A3, 51C1	24482 or 51C	30%-50%	+++
24483	M{vas-int.Dm}ZH-2A, M{3xP3-RFP.attP}ZH-51D	2A3, 51D9	24483 or 51D	30%-50%	+++
24484	M{vas-int.Dm}ZH-2A, M{3xP3-RFP.attP'}ZH-58A	2A3, 58A3	24484 or 58A	30%-50%	+++
24485	M{vas-int.Dm}ZH-2A, M{3xP3-RFP.attP'}ZH-68E	2A3, 68E1	24485 or 68E	30%-50%	+++
24486	M{vas-int.Dm}ZH-2A, M{3xP3-RFP.attP'}ZH-86Fa	2A3, 86E18	24486 or 86Fa	30%-50%	+++
23648**	M{vas-int.B}ZH-102D, M{3xP3-RFP.attP}ZH-86Fb	102D, 86F8	23648**	30%-50%	+++
24487	M{vas-int.Dm}ZH-2A, M{3xP3-RFP.attP'}ZH-96E	2A3, 96E10	24487 or 96E	30%-50%	+++
24488	M{vas-int.Dm}ZH-2A, M{3xP3-RFP.attP}ZH-102D	2A3, 102D	24488 or 102D	30%-50%	+++
23647**	M{vas-int.B}ZH-86Fb, M{3xP3-RFP.attP}ZH-102D	86F8, 102D	23647**	30%-50%	+++
24749	M{vas-int.Dm}ZH-2A, M{3xP3-RFP.attP}ZH-86Fb	2A3, 86F8	24749 or 86Fb	30%-50%	+++
24861	M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-3B}VK00001	2A3, 59D3	24861	9%
24862	M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-9A}VK00005	2A3, 75A10	24862	62%	+++
24863	M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-3B}VK00007	2A3, 82A1	24863	33%
24865	M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-3B}VK00016	2A3, 47C6	24865	17%
24866	M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-9A}VK00019	2A3, 68D2	24866	17%	+++
24867	M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-9A}VK00020	2A3, 99F8	24867	69%
24869	M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-9A}VK00030	2A3, 50E1	24869	23%
24870	M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-3B}VK00031	2A3, 62E1	24870	28%	+++
24871	M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-3B}VK00033	2A3, 65B2	24871	69%	+++
24872	M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-3B}VK00037	2A3, 22A3	24872	41%	+++
24864	M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-9A}VK00013	2A3, 76A2	24864	22%
24868	M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-9A}VK00022	2A3, 57F5	24868	41%
25709	P{nos-phiC31\int.NLS}X, P{CaryP}attP40	X, 25C7	25709	72%
25710	P{nos-phiC31\int.NLS}X, P{CaryP}attP2	X, 68A4	25710	39%
8621	P{CaryP}attP1	55C2-4	8621	66%	+++
8622	P{CaryP}attP2	68A4	8622	62%	+++
9729	PBac{y[+]-attP-3B}VK00010	ch. 2	9729	62%
9734	PBac{y[+]-attP-9A}VK00015	ch. 2	9734	28%
9753	PBac{y[+]-attP-3B}VK00038	5B8	9753	50%	+++
9726	PBac{y[+]-attP-9A}VK00006	1.90E+08	9726	48%	+++
9752	PBac{y[+]-attP-3B}VK00037	22A3	9752	65%	+++
9731	PBac{y[+]-attP-9A}VK00012	25A3	9731	54%
9723	PBac{y[+]-attP-3B}VK00002	2.80E+08	9723	44%
9724	PBac{y[+]-attP-3B}VK00003a	31B1	9724	47%	+++
9730	PBac{y[+]-attP-3B}VK00011	4.00E+05	9730	37%
9733	PBac{y[+]-attP-9A}VK00014	43A1	9733	25%
9735	PBac{y[+]-attP-3B}VK00016	47C6	9735	41%
9728	PBac{y[+]-attP-3B}VK00008	50A3	9728	46%
9747	PBac{y[+]-attP-9A}VK00030	5.00E+02	9747	61%	+++
9736	PBac{y[+]-attP-9A}VK00018	53B2	9736	73%
9740	PBac{y[+]-attP-9A}VK00022	57F5	9740	42%
9722	PBac{y[+]-attP-3B}VK00001	59D3	9722	61%	+++
9748	PBac{y[+]-attP-3B}VK00031	6.20E+02	9748	53%	+++
9750	PBac{y[+]-attP-3B}VK00033	65B2	9750	69%	+++
9754	PBac{y[+]-attP-3B}VK00039	6.70E+05	9754	49%
9737	PBac{y[+]-attP-9A}VK00019	68D2	9737	58%	+++
9741	PBac{y[+]-attP-9A}VK00023	70A2	9741	59%
9751	PBac{y[+]-attP-3B}VK00036	70C4	9751	46%	+++
9725	PBac{y[+]-attP-9A}VK00005	75A10	9725	58%	+++
9732	PBac{y[+]-attP-9A}VK00013	76A2	9732	68%	+++
9739	PBac{y[+]-attP-9A}VK00021	76C5	9739	65%	+++
9746	PBac{y[+]-attP-9A}VK00029	h47-h53	9746	68%
9727	PBac{y[+]-attP-3B}VK00007	82A1	9727	38%
9749	PBac{y[+]-attP-3B}VK00032	85A2	9749	28%
9755	PBac{y[+]-attP-3B}VK00040	87B10	9755	68%	+++
9744	PBac{y[+]-attP-9A}VK00027	8.90E+12	9744	58%	+++
9745	PBac{y[+]-attP-9A}VK00028	92F1	9745	31%
9743	PBac{y[+]-attP-9A}VK00026	96F3	9743	66%
9738	PBac{y[+]-attP-9A}VK00020	99F8	9738	73%	+++
9742	PBac{y[+]-attP-9A}VK00024	101F1	9742	78%
27387	P{attP.w[+].attP}JB37B	37B7	27387	51%
27388	P{attP.w[+].attP}JB38F	38F1	27388	90%
27386	P{attP.w[+].attP}JB53F	53F8	27386	86%
25091	P{attP.w[+].attP}tara[JB89B]	89B8	25091	60%

Note: +++ indicates at least one transgenic line obtained in the test; ** lines no longer in use.