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PhiC31 Integration
System
PhiC31
integration system is a site-specific integration approach which
applies bacteriophage PhiC31 integrase to mediate the
sequence-specific recombination between two largely different
attachment sites (attB and attP). By selection of attB or attP sites
in the genome (landing platforms) and a plasmid containing the
complementary site (an integration vector), transgen can be
integrated on the pre-selected site on the genome.
We thank Dr. Basler lab, Dr. Bellen lab, Dr. Calos lab
and Dr. Perkins lab for allowing our services to
use the attP lines generated in their labs. We also thanks
Bloomington Stock Center for providing all these lines.
Reference:
P{CaryP}
insertions: Groth et al. 2004
PBac{y[+]-attP} insertions: Venken et al. 2006
M{3xP3-RFP.attP}, M{vas-int.B} and
P{nos-phiC31\int.NLS} insertions: Bischof et al. 2007 (see FlyC31 for
additional information the phiC31 integrase system)
M{vas-int.Dm}
insertions: K. Basler and F. Karch, FlyC31
P{attP.w[+].attP} insertions: Bateman et al., 2006
We provide micro-injection service for almost all
the phiC31 lines listed in Bloomington Stock Center (table below).
The service includes injection only service (service A) and extended
service for screening the transformants (service B).
Service A:
injection of 150 to 200 embryos of selected line, sending back 50 to
100 survival larvae with service report.
Service B:
including service A, plus setting up 30 to 50 crosses, sending back
transformants with service report.
The price for
service A: $180 plus $25 handling charge per order. If you need
inject into the lines other than our collection, please provide two
bottles of flies. You will be charged for extra $25 fly
handling fee per order.
The price for
service B: $280 plus $25 handling charge per
order.
The charge
for all the service is non-refundable. However, if there is no
transformants, we provide one free re-injection.
The quality of DNA is critical for successful
transformation. We recommend using Invitrogen Purelink Hipure
Plasmid Fliter Maxiprep kit for large DNA (size over 20kb)
preparation, and Qiagen Midi or Maxi kit for size less than
15kb DNA preparation. Dissolve DNA in DEPC treated water with
concentration over 1.0ug/ul.
RTF standard attP lines collection
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Bloomington Stock
# |
Symbol |
Cytology |
RTF order
number |
| 24480 |
M{vas-int.Dm}ZH-102D,
M{3xP3-RFP.attP}ZH-2A |
102D,
2A3 |
24480 or
2A |
| 24481 |
M{vas-int.Dm}ZH-2A,
M{3xP3-RFP.attP'}ZH-22A |
2A3,
22A2 |
24481 or
22A |
| 24482 |
M{vas-int.Dm}ZH-2A,
M{3xP3-RFP.attP'}ZH-51C |
2A3,
51C1 |
24482 or
51C |
| 24483* |
M{vas-int.Dm}ZH-2A, M{3xP3-RFP.attP}ZH-51D |
2A3,
51D9 |
24483 or 51D |
| 24484 |
M{vas-int.Dm}ZH-2A,
M{3xP3-RFP.attP'}ZH-58A |
2A3,
58A3 |
24484 or
58A |
| 24485* |
M{vas-int.Dm}ZH-2A, M{3xP3-RFP.attP'}ZH-68E |
2A3,
68E1 |
24485 or 68E |
| 24486 |
M{vas-int.Dm}ZH-2A,
M{3xP3-RFP.attP'}ZH-86Fa |
2A3,
86E18 |
24486 or
86Fa |
| 23648** |
M{vas-int.B}ZH-102D,
M{3xP3-RFP.attP}ZH-86Fb |
102D,
86F8 |
23648** |
| 24487* |
M{vas-int.Dm}ZH-2A, M{3xP3-RFP.attP'}ZH-96E |
2A3,
96E10 |
24487 or 96E |
| 24488 |
M{vas-int.Dm}ZH-2A,
M{3xP3-RFP.attP}ZH-102D |
2A3,
102D |
24488 or
102D |
| 23647** |
M{vas-int.B}ZH-86Fb,
M{3xP3-RFP.attP}ZH-102D |
86F8,
102D |
23647** |
| 24749 |
M{vas-int.Dm}ZH-2A,
M{3xP3-RFP.attP}ZH-86Fb |
2A3,
86F8 |
24749 or
86Fb |
| 24861* |
M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-3B}VK00001 |
2A3,
59D3 |
24861* |
| 24862 |
M{vas-int.Dm}ZH-2A,
PBac{y[+]-attP-9A}VK00005 |
2A3,
75A10 |
24862 |
| 24863* |
M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-3B}VK00007 |
2A3,
82A1 |
24863* |
| 24865* |
M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-3B}VK00016 |
2A3,
47C6 |
24865* |
| 24866* |
M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-9A}VK00019 |
2A3,
68D2 |
24866* |
| 24867 |
M{vas-int.Dm}ZH-2A,
PBac{y[+]-attP-9A}VK00020 |
2A3,
99F8 |
24867 |
| 24869* |
M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-9A}VK00030 |
2A3,
50E1 |
24869* |
| 24870 |
M{vas-int.Dm}ZH-2A,
PBac{y[+]-attP-3B}VK00031 |
2A3,
62E1 |
24870 |
| 24871 |
M{vas-int.Dm}ZH-2A,
PBac{y[+]-attP-3B}VK00033 |
2A3,
65B2 |
24871 |
| 24872 |
M{vas-int.Dm}ZH-2A,
PBac{y[+]-attP-3B}VK00037 |
2A3,
22A3 |
24872 |
| 24864* |
M{vas-int.Dm}ZH-2A, PBac{y[+]-attP-9A}VK00013 |
2A3,
76A2 |
24864* |
| 24868 |
M{vas-int.Dm}ZH-2A,
PBac{y[+]-attP-9A}VK00022 |
2A3,
57F5 |
24868 |
| 25709 |
P{nos-phiC31\int.NLS}X,
P{CaryP}attP40 |
X,
25C7 |
25709 |
| 25710* |
P{nos-phiC31\int.NLS}X, P{CaryP}attP2 |
X,
68A4 |
25710* |
| 8621 |
P{CaryP}attP1 |
55C2-4 |
8621 |
| 8622 |
P{CaryP}attP2 |
68A4 |
8622 |
| 9729 |
PBac{y[+]-attP-3B}VK00010 |
ch.
2 |
9729 |
| 9734* |
PBac{y[+]-attP-9A}VK00015 |
ch.
2 |
9734* |
| 9753 |
PBac{y[+]-attP-3B}VK00038 |
5B8 |
9753 |
| 9726 |
PBac{y[+]-attP-9A}VK00006 |
19E7 |
9726 |
| 9752 |
PBac{y[+]-attP-3B}VK00037 |
22A3 |
9752 |
| 9731 |
PBac{y[+]-attP-9A}VK00012 |
25A3 |
9731 |
| 9723 |
PBac{y[+]-attP-3B}VK00002 |
28E7 |
9723 |
| 9724 |
PBac{y[+]-attP-3B}VK00003a |
31B1 |
9724 |
| 9730 |
PBac{y[+]-attP-3B}VK00011 |
40E4 |
9730 |
| 9733 |
PBac{y[+]-attP-9A}VK00014 |
43A1 |
9733 |
| 9735 |
PBac{y[+]-attP-3B}VK00016 |
47C6 |
9735 |
| 9728* |
PBac{y[+]-attP-3B}VK00008 |
50A3 |
9728* |
| 9747 |
PBac{y[+]-attP-9A}VK00030 |
50E1 |
9747 |
| 9736 |
PBac{y[+]-attP-9A}VK00018 |
53B2 |
9736 |
| 9740 |
PBac{y[+]-attP-9A}VK00022 |
57F5 |
9740 |
| 9722 |
PBac{y[+]-attP-3B}VK00001 |
59D3 |
9722 |
| 9748 |
PBac{y[+]-attP-3B}VK00031 |
62E1 |
9748 |
| 9750 |
PBac{y[+]-attP-3B}VK00033 |
65B2 |
9750 |
| 9754 |
PBac{y[+]-attP-3B}VK00039 |
67E4 |
9754 |
| 9737 |
PBac{y[+]-attP-9A}VK00019 |
68D2 |
9737 |
| 9741 |
PBac{y[+]-attP-9A}VK00023 |
70A2 |
9741 |
| 9751 |
PBac{y[+]-attP-3B}VK00036 |
70C4 |
9751 |
| 9725 |
PBac{y[+]-attP-9A}VK00005 |
75A10 |
9725 |
| 9732 |
PBac{y[+]-attP-9A}VK00013 |
76A2 |
9732 |
| 9739 |
PBac{y[+]-attP-9A}VK00021 |
76C5 |
9739 |
| 9746 |
PBac{y[+]-attP-9A}VK00029 |
h47-h53 |
9746 |
| 9727 |
PBac{y[+]-attP-3B}VK00007 |
82A1 |
9727 |
| 9749 |
PBac{y[+]-attP-3B}VK00032 |
85A2 |
9749 |
| 9755 |
PBac{y[+]-attP-3B}VK00040 |
87B10 |
9755 |
| 9744 |
PBac{y[+]-attP-9A}VK00027 |
89E11 |
9744 |
| 9745 |
PBac{y[+]-attP-9A}VK00028 |
92F1 |
9745 |
| 9743 |
PBac{y[+]-attP-9A}VK00026 |
96F3 |
9743 |
| 9738 |
PBac{y[+]-attP-9A}VK00020 |
99F8 |
9738 |
| 9742 |
PBac{y[+]-attP-9A}VK00024 |
101F1 |
9742 |
| 27387 |
P{attP.w[+].attP}JB37B |
37B7 |
27387 |
| 27388 |
P{attP.w[+].attP}JB38F |
38F1 |
27388 |
| 27386 |
P{attP.w[+].attP}JB53F |
53F8 |
27386 |
| 25091 |
P{attP.w[+].attP}tara[JB89B] |
89B8 |
25091 |
Note: ** lines no longer in use, * weak lines, survival rate less than
30%.
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